Compare Fluorescence Microplate Readers from leading suppliers on Biocompare. Conquer Scientific provides laboratories around the world high quality, low-cost pre-owned laboratory instruments. Instrument Configuration: Assay Technology Adapta® & • SpectraMax Mini Multi-Mode Microplate Reader (AccuBlue HiRange only) • SpectraMax® M2/M2e/M3/M4/M5/M5e Multi-Mode Microplate Readers • SpectraMax® Paradigm® Multi-Mode Microplate Reader • FlexStation® 3 Multi-Mode Microplate Reader • Gemini™ EM/XPS Fluorescence Microplate Readers • FilterMax™ F3/F5 Multi-Mode Microplate. 4 software package (Molecular. 3 Pages. 4 Pages. Citations: Dual monochromators increase performance and assay flexibility. 4 Pages. The SpectraMax ® M3, M4, M5, and M5 e Microplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port and 6-well to 384-well microplate reading capability. performs within its specifications. BMG Labtech - FLUOSTAR OMEGA. Click to Contact Seller. SpectraMax Glo Steady-Luc Reporter Assay Kit. Click “Eject Plate Carrier. Instrument Configuration: Assay Technology Adapta® & LanthaScreen® Europium& FlexStation® 3 Benchtop Multi-Mode Microplate Reader : Learn more about the SpectraMax® M5 and the SpectraMax® M5e;. Absorbance was measured at 485 nm with FilterMax F5 Multi-Mode microplate readers and the percentage of viable cells was calculated in relation to cells cultured in pure medium. In addition, the iD5 reader can measure TRF and FP, and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter gene detection with syringe and Western blot detection. After initial incubation, the lysates were incubated with the caspase-1 substrate Ac-YVAD-pNA at 37 °C. SpectraMax® M5/M5e Microplate Reader & FlexStation® 3 Benchtop Multi-Mode Microplate ReaderSpectraMax M-series Microplate readers. » FilterMax™ F5 Multi-Mode Microplate Reader » SpectraMax L Microplate Luminometer ATP + Luciferin + 0 2 Oxyluciferin + AMP + PPi + CO 2 + Light Mg2+ Luciferase Benefits • Sensitive, reliable detection of mycoplasma contamination. Absorbance values were normalized on the control at 24 h and analyzed as being proportional to the number of viable cells. Luminescence (glow) Fluorescence polarization. After adding 100 μl of new ice-cold PBS to the cells, fluorescent intensity was immediately determined using the fluorescent plate reader (FilterMax F3&F5 Multi-Mode Microplate Reader, Molecular Devices). Microplate readers. Unsuccessful migration. Selecting your dsDNA assay kits The family of SpectraMax Quant dsDNA Assay Kits consist of three kits each designed for a specific range of DNA concentrations and optimized for use with florescenceThe cells were washed again, and 90 µL of a substrate solution was added to the wells for 15 min. With optimized reagents, option to operate the. please click here. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time resolved fluorescence assays (TRF). Learn more about the FilterMax™ F5 Multi-Mode Microplate Reader . A Vision for the Future With TTP LabTech. SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers. HTRF-compatible SpectraMax Multi-Mode Readers. With up to five modes of detection, F3/F5 Multi-Mode Microplate Readers support a wide variety of applications in life science research. The data were analyzed using Multi-Mode Analysis software (Molecular Devices, Corp. Once the FilterMax F5 has completely powered on, double-click on the Multi- Mode Analysis Software. The quantity of colorimetric product was continuously monitored for 120 min. SP Bel-Art Petri Dish Plate Reader, Bel-Art Products, a part of SP . All ELISA kits were read using a FilterMax F5 Multi-Mode Microplate Reader (Molecular Devices; San Jose, CA, USA), according to the kit guidelines. Thermo Fisher Scientific. Images were captured using Molecular Devices CloneSelect Imager at a suitable time point post plating. book Page 1 Tuesday, September 7, 2010 6:14 PM. F5 Multi-Mode Microplate Reader. BioStack 4 offers patented plate de-lidding and re-lidding for sensitive cell-based workflows. 4 Pages. For a higher degree of automation, the Automation Vendor Partners Program has streamlined the integration of. The USB adaptor comes with 1 X CD that contains its USB driver. Open SoftMax® Pro software. Devices FilterMax Multi-Mode Microplate Readers. Neurotransmitter Transporter Uptake Assay Kit. 561. By performing Z-height optimization, a lens is adjusted to address low volumes or varied well geometry. Microplate readers from Molecular Devices are designed to provide consistent performance for many years. 005 OD. 4. Synergy HTX and Synergy LX. Molecular Devices’ SpectraMax i3x Multi-Mode Microplate Reader and Thermo Fisher Scientific’s Varioskan LUX reader also perform multiplexing. The user guide also covers the software features, the maintenance procedures, and the technical specifications of the readers. Click Run. Add to my manuals. Multi-mode Microplate Readers. 25. The absorbance of samples at 405 nm (A 405) was determined at room temperature at 20 and/or 60 min after addition of substrate using a FilterMax F5 Multi-Mode Microplate reader (Molecular Devices, San Jose, CA). MetaFluor Software. The absorbance was measured at 450 nm with a FilterMax F3 & F5 multi-mode microplate reader (Molecular Devices, Sunnyvale, CA). Page 4 FilterMax F5 and F3 Multi‐Mode Microplate Readers Unpacking and Setup Guide 5. FilterMax® F3 & F5 Multi-Mode Microplate Readers Molecular Devices LLC Applications: Absorbance, Cell viability, ELISA, Fluorescence,. Manufacturer: Molecular Devices. Page 1 SpectraMax® M3 SpectraMax® M4 SpectraMax® M5 SpectraMax® M5e Multi-Mode Microplate Readers User Guide 0112-0115 G October 2018. BMG LABTECH - CLARIOstar Plus . Page 4 Operator’s Manual Intended Use Statement • The Synergy H1 is a hybrid multi-mode microplate reader. Absorbance mode reads plates up to 0. All configurations offer a triple-mode cuvette port, accurate temperature control, microplate shaking and comprehensive data management using our SoftMax® Pro Microplate Data Representative data from the SpectraMax i3x Multi-Mode Microplate Reader for both MycoAlert and MycoAlert PLUS assays are shown in Figure 3. After 30 min, absorbance of the lysates at 405 nm was measured on a microplate reader (FilterMax F5 Multi-Mode microplate reader). The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time resolved fluorescence assays (TRF). They provide absorbance, fluorescence, glow luminescence, and other read modes for programmable endpoint, kinetic, multiple-wavelength linear scan and area scan measurements. FilterMax® F3 & F5 Multi-Mode Microplate Readers Molecular Devices LLC Applications: Absorbance, Cell viability, ELISA, Fluorescence,. , Sunnyvale, CA, USA) for 30 min and 1 h after dye addition. The FilterMax™ F3 and F5 Multi-Mode Microplate Readers offer a wide set of features at an affordable price-to-performance ratio. The OD 600 was plotted against time using Graphpad Prism 8. This instrument has passed specific verification criteria. You can determine the model and type of your F5 hardware platform through the command line or the Configuration utility. Instrument Configuration: Assay Technology Adapta® & LanthaScreen® EuropiumFlexStation 3 Multi-Mode Microplate Reader A five-mode microplate reader with integrated fluid transfer Key Features • Five-mode reader with wide range of applications • Flexible liquid transfer enables more assay conditions • User-defined pipetting simplifies assay optimization • Instrument and software validation Adapting to biochemical- and cell. Fluorescence was measured using a FilterMax F5 Multi-Mode Microplate Reader (Molecular Devices, Sunnyvale, CA, USA). Molecular Devices technical support is available by phone and e-mail. 00. Background light emission was measured using the luminescence setting on a FilterMax F5 Multi-Mode Microplate Reader (Molecular Techniques). SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers. The present study found competitive inhibition of hMAO-A by ILG along with a similar mode of action against hMAO-B as in rMAO-B. Luminescence was measured on a FilterMax F5 Multi-Mode Microplate Reader (Molecular Devices, LLC, Sunnyvale, CA, USA). 0. Click on the microplate icon in the upper left corner and then click "New" to open a new data file: In the section "Plate1", click on the Settings icon. , GFP expression yields from High Five cells infected with the two viruses were estimated using a FilterMax F5 Multi-Mode Microplate Reader (Molecular Devices, Sunnyvale, CA). Molecular Devices FilterMax F5 Multi-Mode Microplate Reader. A microplate reader that can detect two or more applications is considered a multi-mode plate reader. Learn more about the FilterMax™ F5 Multi-Mode Microplate Reader . The data were analyzed using Multi-Mode Analysis software (Molecular Devices, Corp. This version is compatible with the following instruments: SpectraMax® Mini Multi-Mode Microplate Reader (software version 7. Read Review (1)FlexStation® 3 Multi-Mode Microplate Reader. ** Some compatible filter sets are available for purchase from Life. 2 μM ATP standard was. 40266 Email: drugdiscoverytech@lifetech. Published Dec 16, 2020 | Updated Dec 16, 2020. (M5/M5e) mode reader customized to your specific applications or budgetary needs, while optional capabilities allow you to upgrade with other detection modes at a later time. HERM LB 500 with NaI detector Radio HPLC Detector. The VICTOR Nivo multimode microplate reader is a high-performance filter-based plate reader equipped with all major detection technologies – Alpha, luminescence, etc. Molecular Devices FilterMax™ F5 Multi-Mode Microplate Reader + + Molecular Devices FlexStation. Measurements of Caspase-3/7 Activity. Plate Formats:. Cytokine concentration was determined by plotting the OD or median fluorescence intensity (MFI) of each sample on each cytokine concentration curve and multiplying the result by a dilution. Transfluor Technology. This microplate reader also features BioTek’s Hybrid Technology, which. Read Review (1)With up to five modes of detection, F3/F5 Multi-Mode Microplate Readers support a wide variety of applications in life science research. The ELISA test was performed using the supernatants from the morphological test. FilterMax F5 Multi–Mode microplate reader from Molecular Devices Multi–Mode Analysis software. The optical performance is comparable to a top-of-the-line dedicated Molecular Devices SpectraMax 340PC Microplate Spectrophotometer $ 3,500. The OD value of each tube (230 μL) was measured in a 96-well microplate at 405 nm using a. Depending on the model, Cytation 5 detection modes include fluorescence intensity (FI), fluorescence polarization (FP), time-resolved fluorescence (TRF), luminescence, UV-visible absorbance, imaging, and alpha. Instrumentation and Quality Control. Supplier: Molecular Devices. Learn more about the FilterMax™ F5 Multi-Mode Microplate Reader . After further centrifugation in the same fashion, the supernatant was subjected to subsequent enzymatic reactions. FilterMax F3 & F5 Multi-Mode Microplate Readers: SpectraMax i3x Multi-Mode Microplate Reader: SpectraMax Paradigm Multi-Mode Microplate Detection System: Citations: Figures: Catalog Number: Inquire: FilterMax F3 / FilterMax F5: i3x: Inquire: Price: Description: The SpectraMax® i3 Multi-Mode Detection Platform comes with standard. After 30 min, the reaction was stopped by adding 1 N HCl and absorbance at a wavelength of 490 nm was measured using the FilterMax F5 Multi-Mode microplate reader (Molecular Devices, Corp. Affordability with unmatched assay performance in a multi-mode microplate reader The FilterMax™ F3 and F5 Multi-Mode Microplate Readers provide unmatched. The amount of each HTRA1 protein was. ] Example of document to evaluate if the reader . 7°C and the bottom to 36. The FilterMax™ F3 features absorbance (visible), fluorescence intensity - FI (top reading), and glow luminescence for 96- to 384-well plates. Relative firefly and Renilla luciferase units were measured in a FilterMax F5 Multi-Mode Microplate Reader using the Dual-Luciferase ® Reporter Assay System (Promega) according to the manufacturer’s instructions. SpectraMax Paradigm Microplate Reader General specifications User-upgradeable Multi-Mode Detection Platform Technical specifications Optimized Guaranteed* Fluorescence Intensity (FI) (Fluorescein) 96-well (200 µL) 0. 2. CloneMatrix. Instrument Configuration: Assay Technology Adapta® & LanthaScreen® Europium• FilterMax™ F3/F5 Multi-Mode Microplate Readers Table 1. We recommend you read this assay in dual emission mode, detecting both the emission from the Europium donor fluorophore at 615 nm, and the acceptor fluorophore (at 665 nm for APC. With up to five modes of detection, F3/F5 Multi-Mode Microplate Readers support a wide variety of applications in life science research. Then 100 μL of 2 mol L −1 sulphuric acid was added to stop the enzymatic reaction and the absorbance values were read at 450 nm on a FilterMax F5 Multi-Mode Microplate Reader. Jurkat cells were plated in a 12-well dish at a density of 8 x 10 5 cells in 1 mL media. The measured temperature can be used to provide feedback. FilterMax F5 Multi-Mode Microplate Reader. This document is. 8 Pages. After the absorbance was measured at 450 nm on a FilterMax F5 Multi-Mode Microplate Reader (Molecular Devices, San Jose, CA, USA), the concentrations of the samples were determined by. The performance characteristics of the data reduction software have not been established with any laboratory diagnostic assay. 400. 40266 Email: [email protected]™ F3 and F5 Multimode Microplate Readers, Molecular Devices. 100 fmol 1536-well (8 µL) 0. The SpectraMax® i3 Multi-Mode Detection Platform comes with standard spectral absorbance, fluorescence, and luminescence detection. An extra plate was done with all the serial dispersions for the MgO-NPs (in. The microplates were incubated at 37°C for 24 h, and the growth of bacteria was determined by measuring the optical density (OD) at 630 nm using an ELISA microplate reader (FilterMax F5 multi-mode microplate reader, Molecular Devices, Sunnyvale, CA, USA). 3 Pages. FilterMax F5 Multi-Mode Microplate Reader Includes Excitation Filter Slide #1 (Fluorescence: 485 nm, 535 nm; TRF: 370 nm; FP: 485 nm),. Reaction was stopped by the addition of 100 μl Stop Solution and optical density of each well was determined using a FilterMax F5 Multi-Mode microplate reader and SoftMax Pro 6. Neurotransmitter Transporter Uptake Assay Kit. Transfluor Technology. Optical System: High energy xenon flash lamp. The results are expressed as cell viabilities (%) based on the control condition. 5 mP. Lonza Luminometers Instrument MycoAlert™ Assay MycoAlert™ PLUS Assay Other Bioassays* Notes Lonza Lucetta™ Luminometer + + Cuvette Tailor -made MycoAlert™ Mode Luminometers from Other SuppliersTriplicate reactions were measured every 2. 4 (Molecular Devices, Sunnyvale, CA, USA) at room temperature (fluorimeter settings used were as follows: measurement type, kinetic, 60 cycle, 60-s interval; excitation wavelength, 360 nm;. Turn on the power switch on the back of the instrument. Protease activities were calculated from the slope of the linear portion of the normalized fluorescence vs. A microplate reader is laboratory machine used for the estimation of biochemical or. Page 1 Hybrid Multi-Mode Microplate Reader Synergy ™ Operator’s Manual. Samples (180 μl) were stained with 20 μl of Nile red (2. The results are expressed as cell viabilities (%) based on the control condition. BIO TEK Synergy? 2 Multi-Detection Microplate Reader with Luminescence and Dual Reagent Dispenser SPECTRA MAX 190 MICROPLATE READER (90V TO 240V) WITH CURRENT BMG FLUOSTAR OPTIMA PLATE READER Molecular Devices Filtermax F5 Multi-mode Microplate Reader BioLogic VMP3 Potentiostat Chassis w/9 VMP3-01 Boards potentiostat FilterMax F5 Multi-Mode Microplate Reader. The Analyst®, FlexStation® and SpectraMax® M5/M5e from Molecular Devices have received the LanthaScreen® Certified designation from Invitrogen™ which ensures that these readers are validated to strict standards in instrument se Figure 1: Schematic of a typical workow of the semi solid cloning process. Includes Excitation Filter Slide #1 (Fluorescence: 485 nm, 535 nm; TRF: 370 nm; FP: 485 nm), Emission Filter Slide #1 (Fluorescence: 535. 4 Pages. 5 ml 0. Consult Molecular Devices's entire F3/F5 Multi-Mode Microplate Readers catalogue on MedicalExpo. CloneMatrix. Easily accessible six-position filter slides enable wavelength selection from 340 to 650nm. Typically the system can detect absorbance, luminescence, fluorescence, and even make more specialized fluorescence measurements like time-resolved fluorescence (TRF) and fluorescence polarization (FP). The Analyst®, FlexStation® and SpectraMax® M5/M5e from Molecular Devices have received the LanthaScreen® Certified designation from Invitrogen™ which ensures that these readers are validated to strict standards in instrument se The method is based on the reaction of malondialdehyde (MDA) with thiobarbituric acid (TBA) under conditions of high temperature and acidity that generates a fluorochrome, which is quantified by fluorimetry (F5 Multi-Mode Microplate Reader), with excitation at 515 nm and emission at 553 nm. The 0. SpectraMax Glo Steady-Luc Reporter Assay Kit. 0´ 104 6. #E1910) in a FilterMax F5 Multi-Mode Microplate Reader (Molecular Devices, Sunnyvale, CA). 5 min for 30 min at 340 nm absorbance using a microplate spectrophotometer (Molecular Devices, Sunnyvale, CA, FilterMax F5 Multi-Mode Microplate Reader) and Multi-Mode Analysis Software (Molecular Devices, RRID:SCR_014789), version 3. MetaMorph NX. 1 at 600 nm as determined by FilterMax F5 multi-mode microplate readers (Molecular Devices). VisionMate 96 (Vial Rack Bottom Label 2D Reader) Ziath. The Analyst®, FlexStation® and SpectraMax® M5/M5e from Molecular Devices have received the LanthaScreen® Certified designation from Invitrogen™ which ensures that these readers are validated to strict standards in instrument seF3/F5 Multi-Mode Microplate Readers. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to. FilterMax F3/F5 Multi- Mode Microplate Readers · FilterMax F3 Multi-Mode Microplate Reader Includes Excitation Filter Slide #3 (Absorbance: 405 nm, 450 nm, 492 nm, 620 nm; Fluorescence: of 6 /6. The Analyst®, FlexStation® and SpectraMax® M5/M5e from Molecular Devices have received the LanthaScreen® Certified designation from Invitrogen™ which ensures that these readers are validated to strict standards in instrument seThe OD 600 of the culture in the plate was measured by a FilterMax F5 Multi-Mode Microplate Reader (Molecular Devices) every 20 min over 20 h. ” e. shopping cart 0 items $0. The plate was incubated for 1 hr at room temperature with shaking. The FlexStation 3 Benchtop Multi-Mode Microplate Reader can measure absorbance, fluorescence intensity, fluorescence polarization, luminescence and time-resolved fluorescence. With three modes of detection for UV-Vis absorbance, fluorescence and luminescence, the SpectraMax Mini reader is user upgradeable - from 2 to 3 modes, providing researchers with a versatile instrument that. Molecular Devices FilterMax F5 Multi-Mode Microplate Reader $7,900. 3. Molecular Devices FilterMax F5 Multi-Mode Microplate Reader. Each measurement was repeated at least 3 times, and IC 50 values were calculated by non-linear regression in GraphPad Prism 9. com 8. 3 Pages. Eight replicates of three independent biological samples for each experimental condition (n = 24) were used to validate the collected data. SpectraMax Mini Multi-Mode Microplate Reader. 2 Pages. MetaFluor Software. FlexStation 3 Benchtop Multi-Mode Microplate Reader User Guide Ordering Parts and Accessories To obtain parts and accessories, contact Molecular Devices Customer Service or your authorized service representative. Zebrafish whole-eye mRNA extraction and RT-PCRSearch LabX. In addition, the iD5 reader can measure TRF and FP, and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter gene detection with syringe and Western blot detection. Add to my manuals. After the absorbance was measured at 450 nm on a FilterMax F5 Multi-Mode Microplate Reader (Molecular Devices, San Jose, CA, USA), the concentrations of the samples were determined by extrapolation from a standard curve made in a four-parameter nonlinear regression program in Softmax Pro software (Molecular Devices). Hit enter to search or ESC to close. Configurability is an important aspect of BioTek’s multi-mode readers to provide the most value for laboratory budgets, but the independent optical systems in our multi-mode readers don’t compromise on performance. --> Life sciences. Molecular Devices technical support. ABS Test results. 4 Pages. (PMT) operates in photon counting mode, affording increased sensitivity for red dyes and a linear dynamic range over 6 orders of magnitude. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. FilterMax F3 / FilterMax F5: Inquire: SpectraMax iD3 and iD5: SpectraMax M: Price: Description: Unlimited breadth of user-upgradeable application modules expands research capabilities. Multi-mode Microplate Readers. SpectraMax iD5. This reader provides visible absorbance. SpectraMax Paradigm Multi-Mode Microplate Reader. PHA content was calculated based on the equation (fluorescent intensity/OD 600 × 0. Buffer without protease was used as a blank for background subtraction. SpectraMax® iD5 Multi-Mode Microplate Reader - Five-Mode Hybrid Microplate Reader with Automatic NFC Filter Identification and Western Blot Capability, Molecular Devices. The experiment was carried out with four biological replicates and two technical replicates. The reactions were analyzed in a fluorometer (F5 Multi-Mode Microplate Reader) at 590 nm after a 120-h incubation. --> Life sciences. Search LabX. 8”. Cell growth and viability were assessed using the CCK-8 proliferation assay. ** Some compatible filter sets are available for purchase from Life. com 8. The haemolytic activity was determined in the cell supernatant by optical density readings at 570 nm (FilterMax F5 Multi-Mode Microplate Reader, Molecular Devices). The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. 2. Caspase-3 Activity. Briefly, serial dilutions of TNP were incubated with 200 TCID 50 virus in presence of diethylaminoethyl-dextran and the neutralizing activity was assessed by measuring luciferase activity after 48 h using a FilterMax F5 multi-mode microplate reader (Molecular Devices). 7. Topic. The concentration was expressed as μmol/mg of total protein, based on the use of a standard curve of chloramine T (0-100 μmol/L). Run a variety of luminometric research applications with the Thermo Scientific Luminoskan microplate luminometer, a compact and robust instrument offering versatile plate formats, fast reading speeds and up to three dispensers. The results were calculated using a malondialdehyde (MDA) curve and expressed as nmol MDA/mg protein. The SpectraMax ® iD5 Multi-Mode Microplate Reader is HTRF-certified, with an enhanced TRF detection module and filters optimized for HTRF detection. SpectraMax Quant dsDNA Assay Kits. Learn More. Caspase-3 activity was used as a marker of cell apoptosis and was assessed according to. LabX is a multi-vendor marketplace that connects you with the life science tools and chemistry you need to run your lab. This document is provided to customers. The Analyst®, FlexStation® and SpectraMax® M5/M5e from Molecular Devices have received the LanthaScreen® Certified designation from Invitrogen™ which ensures that these readers are validated to strict standards in instrument seThe OD 405 was measured in a FilterMax™ F5 Multi-Mode Microplate Reader. 4 Pages. A typical HTRF assay uses a europium cryptate as the donor, and the organic fluorophore d2 as the acceptor. Briefly, 2 h after MTS incubation at 37 °C, absorbance at 490 nm was recorded using a microplate reader (FilterMax F5, Multi-Mode Microplate Reader, Molecular Devices, San Jose, MO, USA). The capability of the lixiviated products of the experimental membranes to inhibit the bacterial planktonic growth was estimated from the turbidity of the inoculated culture media at OD λ = 595 nm (filter max F5 multi-mode microplate reader). Supernatant (25 μL), or standard, was added and mixed. • FilterMax™ F3/F5 Multi-Mode Microplate Readers Table 1. The Evans blue dye was extracted from the ears with 1 ml of formamide overnight at 55°C and measured spectrophotometrically at 620 nm (FilterMax TM F5 Multi-Mode Microplate Reader). Method Fluorescence intensity Read Type Endpoint / Kinetic Optics Type Filters Step Label "Linearity Read" Read well C1-F5 Filter Set. SpectraMax i3/i3x and Paradigm readers can be equipped with the HTRF detection cartridge. Cells were incubated at room temperature in the dark for 10 min, then luminescence was detected and quantified with the FilterMax F5 Multi-Mode microplate reader (Molecular Devices, Sunnyvale, CA, USA). There is no programming of protocols or wet chemistry involved –just select the mode(s) you want to test, insert the plate and go. Molecular Devices Filtermax F5 Multi-mode Microplate Reader BioLogic VMP3 Potentiostat Chassis w/9 VMP3-01 Boards potentiostat SYRINGE/VALVE CHAMBER FOR RQF INSTRUMENT quench flow analysis KIN-TEK RAPID QUENCH FLOW INSTRUMENT KIN-TEK RQF-3 VALVE/SYRINGE CHAMBER Biorad Laboratory Real Time PCR System& FlexStation® 3 Benchtop Multi-Mode Microplate Reader : Learn more about the SpectraMax® M5 and the SpectraMax® M5e;. 00. All experiments were performed in triplicate. shopping cart 0 items $0. Molecular Devices’ FilterMax™ F5 Multi-Mode Microplate Reader was tested for compatibility with Life Technologies’ GeneBLAzer® assays. The Molecular Devices FilterMax F5 Multi-Mode Plate Reader was tested for compatibility with Life Technologies’ Z'-LYTE® assays using the Z'-LYTE® Tyr6 kit (PV4122) against. #F5) Methods Standard curves were generated following the assay protocols provided with each kit. It allows the user to choose between monochromator-based optics for flexibility or filter-based optics for sensitivity. MS-3 Laser Bar Code Reader. FilterMax F5 Multi-mode microplate reader. RLUs were determined using a FilterMax F5 multi-mode microplate reader (Beckman Coulter). Add. Imaging by immunofluorescence. 5 million in 2019 and is projected to reach US$ 704. Synergy™ H1 is a flexible monochromator-based multi-mode microplate reader. Instrument Configuration: Assay Technology Adapta® & LanthaScreen® EuropiumThe parasite viability was determined by resazurin assay (0. Multi-mode microplate readers are used in different research fields for the quantification of different biological and chemical assays in a microplate. Instrument Configuration: Assay Technology Adapta® & F3/F5 Multi-Mode Microplate Readers. Page 38 | Getting Started Overview The Cytation 5 is a hybrid multi-mode microplate reader. [The Multi-Mode Validation Plate offers additional testing of TRF, HTRF, and fluorescence polarization read modes on FilterMax™ F3/F5 and SpectraMax® i3x, iD5, and Paradigm® microplate readers. Download Table of Contents. 2 μM ATP standard was. HEK293T cells were harvested 48 h post-transfection in Passive Lysis Buffer (Promega). 00FilterMax F5 Multi-Mode Microplate Reader Includes Excitation Filter Slide #1 (Fluorescence: 485 nm, 535 nm; TRF: 370 nm; FP: 485 nm),. Absorbance values recorded at 490 nm using a microplate reader (FilterMax F5, Multi-Mode Microplate Reader, Molecular Devices) were then analyzed with Excel software (Office, Microsoft) and GraphPad Prism 6 software (La Jolla, CA, USA) to assess statistical significance. Absorbance detection in the UV and visible range (230 nm to 650 nm) Fluorescence intensity; top read and bottom read. F3/F5 Multi-Mode Microplate Readers. A microplate reader that can detect two or more applications is considered a multi-mode plate reader. SpectraMax M2, M2e; SpectraMax M3, M4, M5, M5e; FilterMax™ Multi-Mode Detection Platform. BMG Labtech - THERMOstar. 3 Pages. Learn more about the FilterMax™ F5 Multi-Mode Microplate Reader . HTRF is a versatile TR-FRET technology developed by Cisbio for detecting biomolecular interactions. 6 reviews. Location: San Diego, California, UNITED STATES Member Since: 17th September, 2003 (20 years ago) Current Listings: 667 Shipping Policy: Freight Shipping and Handling: For all orders, Name/Company Name, Address, and Contact Phone Number are required. Molecular Devices FlexStation™ 3 Multi-Mode Microplate Reader w/ One Year Warranty (Svc Contract Available)Find used microplate readers on Machinio to perform a wide range of assays including ELISAs, cell viability assays, nucleic acid quantification, and more. 00 SpectraMax M3, M4, M5, and M5e Multi-Mode Document type: User Manual5016208D 8 Chapter1:Introduction TheFilterMax™ F3Multi-ModeMicroplateReaderandtheFilterMax™F5Multi-Mode MicroplateReaderareidealforabroadrangeofapplications. The user must evaluate this instrument and PC-based software in conjunction with their specific assay(s). Description: The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research. 3 Pages. C1–F5 Filter Set: EX 360/40 nm EM 460/40 nm Optics Position: Gain: Auto Scale to High Wells Scale Well: C1 Scale Value: 30000 Delay after Plate Movement: 100 msec Synergy. ImageXpress Micro 4 High-Content Imaging System. : 28 Sept 12 Page 2 of 20 Setup Guide on the Molecular Devices FilterMax™ F5 Multi-Mode Microplate Reader Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. 2 Pages. All configurations offer a triple-mode cuvette port, accurate temperature control, microplate shaking and comprehensive data management using our SoftMax® Pro Microplate DataFilterMax ™ F5 Multi-Mode Microplate Reader; SpectraMax L Microplate Luminometer; Methods. ScanLater Western Blot Detection System. . [The Multi-Mode Validation Plate offers additional testing of TRF, HTRF, and fluorescence polarization read modes on FilterMax™ F3/F5 and SpectraMax® i3x, iD5, and Paradigm® microplate readers. In the list below the slide number 21, select the positions where the emission filters are installedAfter 30 min, absorbance of the lysates at 405 nm was measured using a microplate reader (FilterMax F5 Multi-Mode microplate reader). Technical specifications Compatible with these Molecular Devices systems Contact Us Phone: +1-800-635-5577 Email: [email protected]. Homogeneous Time-Resolved Fluorescence (HTRF) . The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. Luminescence (glow) Fluorescence polarization. Open SoftMax® Pro software. Biofilm quantification and scanning electron microscopy (SEM) analysis. MD, MPH, Milton J. Microplate readers, multi-mode, FilterMax™ F3 and F5 Supplier: Molecular Devices Description: The FilterMax™ series of multi-mode microplate readers can be operated as standalone instruments or can be integrated seamlessly with laboratory autom. State-of-the-art Multi-Mode Microplate Reader. SoftMax Pro Software, data can be visualized as gray scale, kinetic plots or reaction rates. Instrument Configuration: Assay Technology Adapta® & LanthaScreen® EuropiumThe SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. We specialize in GC, IC, GC/MS, HPLC, LC/MS, Spectrophotometer, and PCR Systems. 40266 Email: [email protected] or higher, standard edition only, no GxP) VersaMax™ Microplate Reader (Serial numbers begining with BNxxxxx and BNRxxxxx are supported) SpectraMax® 190 Absorbance Microplate Reader (Serial numbers begining with. In the list below the slide number 21, select the positions where the emission filters are installedF3/F5 Multi-Mode Microplate Readers. Preconfigured HTRF protocols in SoftMax ® Pro Software 7. SpectraMax Paradigm Multi-Mode Microplate Detection System from Molecular Devices, LLC. Download Table of Contents. The medium was then removed and the cells washed once with 200 μl of ice-cold phosphate buffered saline (PBS). At day 4 P. 3 Pages. FilterMax F5 Multi-Mode Microplate Reader. Molecular Devices’ FilterMax™ F5 Multi-Mode Microplate Reader was tested for compatibility with Life Technologies’ GeneBLAzer® assays. ScanLater Western Blot Detection System. Learn more about the FilterMax™ F5 Multi-Mode Microplate Reader . F3/F5 Multi-Mode Microplate Readers The FilterMax Series of microplate readers includes: FilterMax F3 Multi-Mode Microplate Reader. Learn more about the FilterMax™ F5 Multi-Mode Microplate Reader . The OD 600 was plotted against time using Graphpad Prism 8. SpectraMax Paradigm Multi-Mode Safety Guide; SpectraMax Paradigm Multi-Mode Unpacking Guide; SpectraMax Paradigm Multi-Mode. 3 Pages. 5. 3 Pages. MetaMorph NX. SpectraMax M-series Microplate readers. 00. The SpectraMax iD5 reader uses the HTRF detection system, which includes an Enhanced TRF Module and filters for excitation and emission. Fluorescence was measured with excitation and emission wavelengths of 530 and 590 nm, respectively, on a FilterMax F5 Multi-Mode microplate reader (Molecular Devices, Corp. Learn more about the FilterMax™ F5 Multi-Mode Microplate Reader . FilterMax F5 Multi-Mode Microplate Reader. Multi-Mode Detection Platform Overview The FilterMax™ F3 and FilterMax™ F5 Multi-Mode Detection Platforms are computer-controlled multi-mode detectors capable of performing absorbance, luminescence, and fluorescence intensity measurements on microplate samples (Figure 1-1). SpectraMax i3/i3x Multi-Mode Microplate Reader SoftMax Pro Software 0 500 1000 1500 2000 2500 3000 0 1´ 106 2´ 106 3´ 106 dsDNA (pg/we l) R F U 02 4 6 8 1 0 2. SpectraMax Glo Steady-Luc Reporter Assay Kit. the luminescence reading was taken on a FilterMax F5 Multi-Mode. Add to cart. Instrument Configuration: Assay Technology Adapta® & FilterMax F5 Multi-Mode Microplate Reader. Category 2 and 3 microplate readers use gradient heating as well. Absorbance was measured at 450 nm using a FilterMAx F5 Multi-Mode Microplate Reader and EX2 filter after a 2-hour incubation at 37°C with 10 μL of CCK-8 per 100 μL of media. SpectraMax Quant dsDNA Assay Kits. 4 Pages. 0´ 104 8. Absorbance was detected from the bottom using the FilterMax F5 multimode microplate reader (Molecular Devices, Sunnyvale, CA) at excitation/emission wavelengths of 485 nm/535 nm. Please click on the links below to view the Microplate Reader Comparison Tables. Consult Molecular Devices's entire F3/F5 Multi-Mode Microplate Readers catalogue on MedicalExpo. SpectraMax iD5. 3 Pages. Sign In Upload. They provide absorbance, fluorescence, glow. A microplate reader (also called sometimes microtiter plate reader) is a high-sensitivity instrument designed to analyze samples contained in microplate wells. F3 F5 F3-SC F5-SC F3 F5. The monochromator optics uses a third generation quadruple grating design that allows working at any excitation or emission wavelength with a 1 nm step. The absorption was measured in a spectrophotometer ultraviolet–visible (UV–Vis) Filter Max F5 multi-mode microplate reader; a wavelength of 595 nm was taken as the standard value for bacteria. Microplate Reader Comparison Charts. All experiments were performed in triplicate. ” 3. The absorbance was read with a spectrophotometer (Filter Max F5 Multi-Mode Microplate Reader, Molecular Devices, San Jose, CA, USA) at a wavelength of 595 nm. Recommended Optics • Use the touchscreen to easily set up protocols, run experiments or view tutorial videos • Automatically identify filter sets using NFC functionality, simplifying workflows and eliminating confusion • Perform western blot detection • Push data to workstations with QuickSync, eliminating the need to retrieve data from the instrument SpectraMax iD5 Multi-Mode Microplate Reader Five-mode. Owner, Zeckhausen. Important things to consider when purchasing a multimode microplate reader. Measuring Time: Reading Times (Flying Mode 1 flash): 9 sec (96), 16 sec (384) Wavelength Range:FilterMax F5 Multi-Mode Microplate Readers, 96 to 384 Well or 6 to 1536 Well, Molecular Devices, LLC (CAT#: STEM-LE-0509-LC) Mithras LB 940 Multimode Microplate Reader, 200-1000 nm Spectral Range Absorbance, BERTHOLD TECHNOLOGIES GmbH & Co. The 50% cytotoxic concentration (CC 50) of F5-2a was calculated. The donor and acceptor can be used to label a variety of biomolecules, for. See More Information. Trinean Dropsense 96 Polychromatic. SpectraMax iD5. Fred A. A Filter Max F5 Multi-Mode Microplate Reader was used to measure absorbance at 595 nm. Additionally, user-installable options allow the SpectraMax i3 System to grow with your changing application needs to fulfill and go beyond the standard reader applications, protecting your intial investment.